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recombinant mouse tgf β1  (R&D Systems)


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    R&D Systems recombinant mouse tgf β1
    Recombinant Mouse Tgf β1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 422 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tgf+%CE%B2/Recombinant+Mouse+TGF-beta+1+Protein/pmc12977192-98-3-6
    Average 96 stars, based on 422 article reviews
    recombinant mouse tgf β1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Adipocyte FGF21 Signaling Defect Aggravated Adipose Tissue Inflammation in Gestational Diabetes Mellitus.
    Article Snippet: Plates were coated with 2 μg/mL anti-CD3 mAb (Clone# 145-2C11, Cat#553057, BD Biosciences, New York, NY, USA) and 2 μg/mL anti-CD28 (Clone# 37.51, Cat#553294, BD Biosciences) at 37 ◦C for 2 h and washed with PBS. .. Cells were also stimulated with 100 U/mL Recombinant Mouse IL-2 (Cat#402-ML, R&D systems) and 3 ng/mL Recombinant Mouse TGF-β (Cat#240-B, R&D systems), in the presence or absence of treatments (FGF21,100 μM or PGE2, 100 nM) for the indicated time. ..

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Article Title: Adipocyte FGF21 Signaling Defect Aggravated Adipose Tissue Inflammation in Gestational Diabetes Mellitus
    Article Snippet: Plates were coated with 2 μg/mL anti-CD3 mAb (Clone# 145-2C11, Cat#553057, BD Biosciences, New York, NY, USA) and 2 μg/mL anti-CD28 (Clone# 37.51, Cat#553294, BD Biosciences) at 37 °C for 2 h and washed with PBS. .. Cells were also stimulated with 100 U/mL Recombinant Mouse IL-2 (Cat#402-ML, R&D systems) and 3 ng/mL Recombinant Mouse TGF-β (Cat#240-B, R&D systems), in the presence or absence of treatments (FGF21,100 μM or PGE2, 100 nM) for the indicated time. ..

    Article Title: Effects of Graphene Quantum Dots on Renal Fibrosis Through Alleviating Oxidative Stress and Restoring Mitochondrial Membrane Potential
    Article Snippet: NIH3T3 (ATCC: CRL‐1658) mouse fibroblasts were cultured in DMEM high glucose media supplemented with 10% FBS and 1% streptomycin. .. NIH3T3 fibroblasts were exposed to recombinant mouse TGF‐β (2 ng mL −1 ; R&D systems, Table , Supporting Information) and GQDs (0.25 or 0.5 μg mL −1 ) for 48 h. ..

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury.
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Article Title: A combination of 5/6-nephrectomy and unilateral ureteral obstruction model accelerates progression of remote organ fibrosis in chronic kidney disease.
    Article Snippet: .. After overnight culture, the cells were grown to 70%– 80% confluence and treated with 1 mM indoxyl sulfate potassium salt (Santa Cruz Biotechnology), 10 ng/mL recombinant human TGF- β (R&D Systems, Inc.), or recombinant mouse TGF- β (R&D Systems, Inc.). ..

    Article Title: CKD-Induced Oxidative Twitch Muscle Atrophy Is Mediated by TGF- β
    Article Snippet: .. In experiments with daily TGF- β administration, recombinant mouse TGF- β (R&D Systems, MN) was injected intraperitoneally at a dose of 50 ng/d, while mice in the normal group received equivalent saline injections. ..

    Article Title: Effects of Graphene Quantum Dots on Renal Fibrosis Through Alleviating Oxidative Stress and Restoring Mitochondrial Membrane Potential.
    Article Snippet: NIH3T3 (ATCC: CRL-1658) mouse fibroblasts were cultured in DMEM high glucose media supplemented with 10% FBS and 1% streptomycin. .. NIH3T3 fibroblasts were exposed to recombinant mouse TGF-β (2 ng mL−1; R&D systems, Table S4, Supporting Information) and GQDs (0.25 or 0.5 μg mL−1) for 48 h. Assessment of Renal Function: After 24 h urine collection, urine protein and creatinine (Cr) concentrations were measured, and the urine protein/Cr ratio (mg mg−1) was calculated. ..

    Activation Assay:

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury.
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    In Vitro:

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury.
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Cell Culture:

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury.
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Positive Control:

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Article Title: Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury.
    Article Snippet: .. To measure accelerated HSC activation in vitro, mHSCs were cultured for 7 days and challenged with 10 ng/mL of recombinant mouse TGF –β (R&D Systems #7666-MB) as a positive control of HSC activation, or recombinant mouse Wnt3a (R&D Systems #1324-WN) or Wnt5a (R&D Systems #645-WN), DKK-1 (R&D Systems #5897-DK) on day 5. ..

    Injection:

    Article Title: CKD-Induced Oxidative Twitch Muscle Atrophy Is Mediated by TGF- β
    Article Snippet: .. In experiments with daily TGF- β administration, recombinant mouse TGF- β (R&D Systems, MN) was injected intraperitoneally at a dose of 50 ng/d, while mice in the normal group received equivalent saline injections. ..

    Saline:

    Article Title: CKD-Induced Oxidative Twitch Muscle Atrophy Is Mediated by TGF- β
    Article Snippet: .. In experiments with daily TGF- β administration, recombinant mouse TGF- β (R&D Systems, MN) was injected intraperitoneally at a dose of 50 ng/d, while mice in the normal group received equivalent saline injections. ..



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    Image Search Results


    IGLC3 - tumor cells promote SPP1 + macrophage polarization. (A) TSNE of 5 macrophage cell clusters in scRNA-seq of colorectal tumor tissues, including C1QC&LYVE1 + macrophages, C1QC + macrophages, C1QC&ISG15 + macrophages, ISG15 + macrophages and SPP1 + macrophages. (B) Heatmap of top differential gene expression between each macrophage subtypes. (C) Dot plot of inflammatory macrophages marker genes in each macrophage subtype. (D) The developmental trajectory between macrophage subtypes by monocle2 analysis. (E) Schematic diagram of macrophage and tumor cells co-culture model. (F) Heatmap of C1QC, LYVE1, SPP1 and ISG15 at mRNA level in THP-1 derived M0 macrophages co-cultured with vector and IGLC3 overexpressed HCT116/SW480 cells. The PBS treated THP-1 cells were set as control. (G) mRNA expression of CD206, IL-10 and Arg1 in patients derived SPP1 + macrophages (tumor tissues), and peripheral blood mononuclear cells derived M0 and M2 macrophages (blood). (H) Immunostaining of CD68 and SPP1 in patients derived colorectal tumor tissues. The CD68 and SPP1 + cells distribution was compared between patients with stage I~II (low stage) or stage III~IV (high stage), with metastasis or not, and responding to chemotherapy or not. (I) Volcano plot of differentially expressed cytokine-related genes between IGLC3 + and IGLC3 - tumor cells identified from our scRNA-seq dataset ( GSE166555 ). (J, K) Elisa of TGF-β and CXCL3 in supernatant of vector and IGLC3 overexpressed HCT116/SW480 cells. (L) mRNA expression of SPP1, ISG15, CD80 and CD68 in THP-1 derived M0 macrophages treated with TGF-β (10 ng/ml) and CXCL3 (50 ng/ml). All experiments were independently repeated at least three times with consistent results. Data are presented as mean ± SD, and statistical analyses were performed using t test. *p<0.05, **p<0.01.

    Journal: Frontiers in Immunology

    Article Title: IGLC3 - tumor cells drive chemoresistance in colorectal cancer by polarizing SPP1 + macrophages via the CD44-Wnt-BTF3 axis

    doi: 10.3389/fimmu.2026.1731216

    Figure Lengend Snippet: IGLC3 - tumor cells promote SPP1 + macrophage polarization. (A) TSNE of 5 macrophage cell clusters in scRNA-seq of colorectal tumor tissues, including C1QC&LYVE1 + macrophages, C1QC + macrophages, C1QC&ISG15 + macrophages, ISG15 + macrophages and SPP1 + macrophages. (B) Heatmap of top differential gene expression between each macrophage subtypes. (C) Dot plot of inflammatory macrophages marker genes in each macrophage subtype. (D) The developmental trajectory between macrophage subtypes by monocle2 analysis. (E) Schematic diagram of macrophage and tumor cells co-culture model. (F) Heatmap of C1QC, LYVE1, SPP1 and ISG15 at mRNA level in THP-1 derived M0 macrophages co-cultured with vector and IGLC3 overexpressed HCT116/SW480 cells. The PBS treated THP-1 cells were set as control. (G) mRNA expression of CD206, IL-10 and Arg1 in patients derived SPP1 + macrophages (tumor tissues), and peripheral blood mononuclear cells derived M0 and M2 macrophages (blood). (H) Immunostaining of CD68 and SPP1 in patients derived colorectal tumor tissues. The CD68 and SPP1 + cells distribution was compared between patients with stage I~II (low stage) or stage III~IV (high stage), with metastasis or not, and responding to chemotherapy or not. (I) Volcano plot of differentially expressed cytokine-related genes between IGLC3 + and IGLC3 - tumor cells identified from our scRNA-seq dataset ( GSE166555 ). (J, K) Elisa of TGF-β and CXCL3 in supernatant of vector and IGLC3 overexpressed HCT116/SW480 cells. (L) mRNA expression of SPP1, ISG15, CD80 and CD68 in THP-1 derived M0 macrophages treated with TGF-β (10 ng/ml) and CXCL3 (50 ng/ml). All experiments were independently repeated at least three times with consistent results. Data are presented as mean ± SD, and statistical analyses were performed using t test. *p<0.05, **p<0.01.

    Article Snippet: The following reagents were used: recombinant human TGF-β, CXCL3, fibronectin (FN), SPP1, 5-fluorouracil (5-Fu), and oxaliplatin (Oxa) (all from R&D Systems, USA).

    Techniques: Gene Expression, Marker, Co-Culture Assay, Derivative Assay, Cell Culture, Plasmid Preparation, Control, Expressing, Immunostaining, Enzyme-linked Immunosorbent Assay